Quantification of Mitochondrial DNA Heteroplasmy by Temporal Temperature Gradient Gel Electrophoresis

Quantification of mitochondrial DNA heteroplasmy by temporal temperature gradient gel electrophoresis.

Detection of mitochondrial DNA mutations by temporal temperature gradient gel electrophoresis.

BACKGROUND A unique requirement for the molecular diagnosis of mitochondrial DNA (mtDNA) disorders is the ability to detect heteroplasmic mtDNA mutations and to distinguish them from homoplasmic sequence variations before further testing (e.g., sequencing) is performed. We evaluated the potential utility of temporal temperature gradient gel electrophoresis (TTGE) for these purposes in patients ...

Rapid analysis of mitochondrial DNA heteroplasmy in diabetes by gel-microchip electrophoresis.

Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).

18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...

comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis profiles as a tool for the differentiation of candida species

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...